An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice
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چکیده
منابع مشابه
An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice
Targeted mutant mice generated on a C57BL/6 background are powerful tools for analysis of the biological functions of genes, and gene targeting technologies using mouse embryonic stem (ES) cells have been used to generate such mice. Recently, a bacterial artificial chromosome (BAC) recombineering system was established for the construction of targeting vectors. However, gene retrieval from BACs...
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derivation of es-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2017
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.16-0075